Comparison of K-Sol and M-K medium for cornea storage: results of penetrating keratoplasty in rabbits.

Successful penetrating keratoplasty in an infant after extended storage of infantile donor cornea.

K-Sol corneal preservation at room temperature.

K-Sol, a recently developed corneal storage medium that contains purified chondroitin sulphate in tissue culture medium (TC 199), is capable of preserving corneal tissue for 14 days at 4 degrees C. To study the effect of tissue storage in K-Sol at room temperature we preserved rabbit corneas in K-Sol and M-K medium for three or seven days at 25 degrees C. All of the corneal endothelial sheets w...

Corneal wound healing: Immunohistological features of extracellular matrix following penetrating keratoplasty in rabbits.

PURPOSE To study the distribution and the constituents of the extracellular matrix in the cornea during wound healing following penetrating keratoplasty (PKP). METHODS Penetrating keratoplasty (PKP) was performed on albino rabbit eyes, and immunohistochemical techniques were used to determine the distribution of types I, III, IV collagens, large proteoglycans, chondroitin 6-sulfate, chondroit...

In vitro specular microscope perfusion of M-K- and moist chamber-stored human corneas.

Human donor corneas were stored in McCarey-Kaufman (M-K) medium for 3 to 6 days; then they were mounted in the specular microscope, and the endothelial surface perfused with a glutathione-bicarbonate-Ringer solution. During storage, the corneal thickness increased 37% above an assumed normal of 0.520 mm. The corneas did not have sufficient active thinning to be accepted as viable when tested by...

Hypothermic preservation of corneas in a hyperkalaemic solution (CPTES): II. Extended storage in the presence of chondroitin sulphate.

Periods of preservation for donor corneas, even for short times, are necessary to facilitate optimum conditions in penetrating keratoplasty. However, current techniques for corneal storage at low temperatures may not provide optimal conditions for maintaining tissue integrity. In particular, the ionic composition of the storage medium has received little attention since it has been assumed thro...